Journal: Cell reports

Article Title: Genetic- and diet-induced ω -3 fatty acid enrichment enhances TRPV4-mediated vasodilation in mice

doi: 10.1016/j.celrep.2022.111306

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Endothelial Cell Growth Medium MV 2 , Promo Cell , C-22121.

Techniques: Recombinant, Protease Inhibitor, Staining, Western Blot, Membrane, Protein Extraction, Plasmid Preparation, Software, Imaging, Microscopy

Journal: Molecular medicine reports

Article Title: Intraperitoneal injection of thalidomide alleviates early osteoarthritis development by suppressing vascular endothelial growth factor expression in mice.

doi: 10.3892/mmr.2018.8980

Figure Lengend Snippet: Figure 2. mRNA expression levels of VEGF and MMP‑13 in the knee articular cartilage of mice among the Sham, Dmm and Dmm+Th groups (n=4 in each group). (A) Relative mRNA expression levels of VEGF in the medial articular cartilage. (B) Relative mRNA expression levels of MMP‑13 in the medial articular cartilage. The values are presented as the mean ± standard deviation. *P<0.05 compared with the Sham group; #P<0.05 compared with the Dmm group. Dmm, destabilization of the medial meniscus; MMP‑13, matrix metalloproteinase‑13; Th, thalidomide; VEGF, vascular endothelial growth factor.

Article Snippet: An ELISA kit of VEGF (E-EL-M1292c) was purchased from Elabscience Biotechnology Co., Ltd., Wuhan, China.

Techniques: Expressing, Standard Deviation

Journal: Molecular medicine reports

Article Title: Intraperitoneal injection of thalidomide alleviates early osteoarthritis development by suppressing vascular endothelial growth factor expression in mice.

doi: 10.3892/mmr.2018.8980

Figure Lengend Snippet: Figure 3. Immunohistochemical analysis of VEGF expression in the knee articular cartilage of mice among the Sham, Dmm and Dmm+Th groups (n=4 in each group). (A) Immunohistochemistry staining of VEGF in the articular cartilage of the medial tibial plateau (magnification, x400, scale bar=100 µm). (B) Quantification of VEGF positive cells, based on the results of immunohistochemistry staining. The values are presented as the mean ± standard deviation. *P<0.05 compared with the Sham group; #P<0.05 compared with the Dmm group. Dmm, destabilization of the medial meniscus; Th, thalidomide; VEGF, vascular endothelial growth factor.

Article Snippet: An ELISA kit of VEGF (E-EL-M1292c) was purchased from Elabscience Biotechnology Co., Ltd., Wuhan, China.

Techniques: Immunohistochemical staining, Expressing, Immunohistochemistry, Staining, Standard Deviation

Journal: Molecular medicine reports

Article Title: Intraperitoneal injection of thalidomide alleviates early osteoarthritis development by suppressing vascular endothelial growth factor expression in mice.

doi: 10.3892/mmr.2018.8980

Figure Lengend Snippet: Figure 5. ELISA analysis of serum VEGF concentration of mice among the Sham, Dmm and Dmm+Th groups (n=8 in each group). The values are presented as the mean ± standard deviation. *P<0.05 compared with the Sham group; #P<0.05 compared with the Dmm group. Dmm, destabilization of the medial meniscus; Th, thalidomide; VEGF, vascular endothelial growth factor.

Article Snippet: An ELISA kit of VEGF (E-EL-M1292c) was purchased from Elabscience Biotechnology Co., Ltd., Wuhan, China.

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Standard Deviation

Journal: Biological Chemistry

Article Title: Peritumoral administration of GPI-anchored TIMP-1 inhibits colon carcinoma growth in Rag-2 γ chain-deficient mice

doi: 10.1515/bc.2009.098

Figure Lengend Snippet: Figure 2 The GPI-anchored TIMP-1 suppresses proliferation of SW480 cells and inhibits tumor invasion. The effect of cell surface engineering with TIMP-1-GPI on SW480 invasion through the Matrigel basement membrane model was assessed. Optimal invasion of the SW480 cells in response to 10% FCS, 4 ng/ml EGF, or 4 ng/ml VEGF were set to ‘zero’, and the 100% inhibition value was set to the migration level observed with untreated SW480 cells (Djafarzadeh et al., 2004). 5=103 SW480 cells per 100 ml medium were cultured in 96-well microtiter plates for 24 h under standard conditions to yield firmly attached and stably growing cells (Djafarzadeh et al., 2006). The SW480 cells were then pretreated with 2, 4, 6, 8, 10, 12, and 14 ng/ml of TIMP-1-GPI or 14 ng/ml of rhTIMP-1 as described previously (Djafarzadeh et al., 2006). After 1 h the cells were washed. (A) The effect of increasing levels of TIMP-1-GPI or rhTIMP-1 control protein on the proliferation of SW480 was measured using an MTT assay (Djafarzadeh et al., 2006). MTT was added after 24 h, 48 h, or 72 h as indicated. (B) The effect on the ability of SW480 to invade Matrigel was measured using a modified Boyden chamber as described earlier (Djafarzadeh et al., 2004, 2006).

Article Snippet: The effect of TIMP-1-GPI vs. rhTIMP-1 treatment on the ability of SW480 cells to invade Matrigel was measured using induced migration to fetal calf serum (FCS) (Biocrom, Berlin, Germany), vascular endothelial growth factor (VEGF) (R&D systems, Minneapolis, USA), and EGF (Biochrom, Berlin, Germany) using a modified Boyden chamber (Djafarzadeh et al., 2004, 2006).

Techniques: Membrane, Inhibition, Migration, Cell Culture, Stable Transfection, Control, MTT Assay, Modification

Journal: PloS one

Article Title: Embedding synthetic microvascular networks in poly(lactic acid) substrates with rounded cross-sections for cell culture applications.

doi: 10.1371/journal.pone.0073188

Figure Lengend Snippet: Figure 3. Seeding and culture of bovine aortic endothelial cells (BAECs) throughout PLA microchannel networks. (a) Confocal microscopy shows that the interior walls of a 200 mm diameter straight circularized microchannel can be uniformly seeded with endothelial cells that subsequently are confluently cultured in a monolayer lining the channel wall. (b) Fluorescent images show BAECs survive and maintain their morphology after 5 days in the straight circularized microchannel (bar, 50 mm). (c) BAECs seeded in four generations of branched microchannel network with diameters extending below 50 mm uniformly cover all channel walls and maintain viability after 3 days of culture (bar, 50 mm). doi:10.1371/journal.pone.0073188.g003

Article Snippet: Bovine Aortic Endothelial Cells (BAECs; Cell Applications, Inc., CA) obtained as a kind gift from Dr. Mariah Hahn at Texas A&M University were resuspended at 56106 cells mL–1 medium for 500 mm diameter channels or at 26107 cells mL–1 medium for 100 mm diameter and branched channels.

Techniques: Confocal Microscopy, Cell Culture